Methods : formulas of the expected pooled sample size and the power of tests were deduced while controlling the probability of type and type errors 方法：在控制两类错误概率的前提下，推导两种方法的期望检测次数和检验功效的数学表达式。
2.
The optimal pool sizes of the 2 approaches were given by minimizing the expected pooled sample size ; computer simulation was used to verify the outcomes 对最大期望检测次数进行数学优化，求解最优混合样本的大小，并通过计算机模拟对有关结果进行验证。
3.
Objective : to provide a minimum sample size approach and a sequential sampling approach for testing whether the sporozoite rate has exceeded the critical level of malaria epidemics using the pool sampling method 摘要目的：给出基于混合样本的最小检测次数法和序贯检验法，以检验子孢子阳性率是否超过导致疟疾流行的临界水平。
4.
Conclusion : the optimal pool size in the present study can obtain satisfactory testing power ( type and type errors are both lower than 5 % ) and can effectively decrease the pooled sample size 结论：利用本文给出的最优混合样本和检测步骤，在获得比较满意的检验功效（两类错误概率均低于5 % ）的同时，可以有效降低检测次数。
